Model of vascularized composite allotransplantation (VCA) involving orthotopic engraftment of hind limbs from BALB/c to C57BL/6 mice.
Transplantation
Our lab undertakes various clinical and experimental transplant projects. The experimental work, headed by Dr. Liqing Wang, involves cardiac, renal and islet transplantation models in inbred mice includes. Dr. Wang has recently added murine heterotopic and orthotopic limb transplantation, and murine orthotopic lung transplantation, to our transplant repertoire.
Dr. Liqing Wang demonstrates his advanced microsurgical skills by undertaking mouse orthotopic lung transplantation with direct suturing of bronchi, and pulmonary arteries and veins, rather than using a standard cuff technique, in the video below (www.youtube.com/watch?v=1ThYAicOv1o). This advanced technique results in significant shortening of surgical time and improved survival and function.
Selected Publications
Xu H, Dahiya S, Wang L, Akimova T, Han R, Zhang T, Zhang Y, Qin L, Levine MH, Levin LS, Hancock WW. Utility of IL-2 complexes in promoting the survival of murine orthotopic forelimb vascularized composite allografts. Transplantation (In press)
Targeting Sirtuin-1 prolongs murine renal allograft survival and function. Levine MH, Wang Z, Xiao H, Jiao J, Wang L, Bhatti TR, Hancock WW, Beier UH. Kidney Int. 2016 May;89(5):1016-26. Free PMC Article
Differing effects of rapamycin or calcineurin inhibitor on T-regulatory cells in pediatric liver and kidney transplant recipients. Akimova T, Kamath BM, Goebel JW, Meyers KE, Rand EB, Hawkins A, Levine MH, Bucuvalas JC, Hancock WW. Am J Transplant. 2012 Dec;12(12):3449-61. Free PMC Article
Sirtuin-1 targeting promotes Foxp3+ T-regulatory cell function and prolongs allograft survival. Beier UH, Wang L, Bhatti TR, Liu Y, Han R, Ge G, Hancock WW. Mol Cell Biol. 2011 Mar;31(5):1022-9. Free PMC Article
Regulatory T cell expression of herpesvirus entry mediator suppresses the function of B and T lymphocyte attenuator-positive effector T cells.T Tao R, Wang L, Murphy KM, Fraser CC, Hancock WW. J Immunol. 2008 May 15;180(10):6649-55.
Programmed cell death 1 (PD-1) and its ligand PD-L1 are required for allograft tolerance. Wang L, Han R, Hancock WW. Eur J Immunol. 2007 Oct;37(10):2983-90.
Permanent survival of fully MHC-mismatched islet allografts by targeting a single chemokine receptor pathway. Wang L, Han R, Lee I, Hancock AS, Xiong G, Gunn MD, Hancock WW.
J Immunol. 2005 Nov 15;175(10):6311-8. Recruitment of Foxp3+ T regulatory cells mediating allograft tolerance depends on the CCR4 chemokine receptor. Lee I, Wang L, Wells AD, Dorf ME, Ozkaynak E, Hancock WW. J Exp Med. 2005 Apr 4;201(7):1037-44.
